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Incubations were initiated by the addition of test amide (final concentration 0.5 M, 0.25% DMSO) and baseline (t 0 ) samples were taken immediately and quenched in ice cold acetonitrile with 1% formic acid

Indeed, clientized Thr1273Glu S heptapeptide demonstrated binding to purified WD40 with a K D of 2.30 0.23 M (Table 1, Fig

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We used 50 ng of cDNA for the PCR template, real-time PCR was conducted using thermal cycler Quant Studio 3 (Thermo Fisher Scientific, Waltham, MA, USA) and thermal cycling conditions were: 2 min at 50C and 10 min at 95C followed by 40 cycles at 9C for 15 s and at 60C for 1 min (37)

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