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The RNA content was estimated spectrophotometrically (Eppendorf BioSpectrometr basic, Hamburg, Germany), and its purity and integrity were checked on a 1.5% (w/v) agarose gel

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Because they act through distinct mechanisms at different levels of the axis, some practitioners describe them as complementary: Ipamorelin supports systemic endocrine signaling, while IGF-1 DES delivers localized anabolic stimulation at the receptor level