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Animals were housed and bred in a pathogen-free animal facility at the Keck School of Medicine, University of Southern California (USC), in accordance with protocols approved by the Institutional Animal Care and Use Committee

Key directions for subsequent research include elucidating the synergistic dynamics within the target network, optimizing therapeutic dosing, and assessing palmatines clinical translation potential, drug interaction profiles, human pharmacokinetics, and safety profile

Erastin induces the expression changes in ferroptosis markers and inhibits melanin synthesis in melanocytes, which can be attenuated by N-acetyl-L-cysteine (NAC) pretreatment or post-treatment in vitro

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Therefore, TMAO can aggravate atherosclerosis formation by affecting the metabolism of bile acid and cholesterol