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The referenced comparison trial included a daily 500-calorie deficit, 150 minutes of weekly exercise, and in-person counseling every four weeks - a fundamentally different protocol with different study populations, different variables, and different timeframes

The protein samples were digested with trypsin (trypsin: protein = 1:20, w/w) at 37 C

On the other hand, Fats are transformed into fatty acids while proteins into their simpler building blocks called amino acids

In these situations, serial HCG tests are performed to monitor the trend of HCG levels

The statistical significance indicated for ( a - c ) represents p value cutoff = 0.05, fold change cutoff = 1.5 and Welch t -test (FDR corrected)