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Amara SG, Jonas V, Rosenfeld MG, Ong ES, Evans RM

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Use only medical-grade bacteriostatic water or sterile water for injection and 0.6% acetic acid water for the specific peptides that need it

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In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min