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To compare the expression of these genes in response to H 2 O 2 , we measured by qPCR the transcriptional level of each one in the pneumococcal R801strain (derivate of the R6 strain) [31] exposed at 20 mM H 2 O 2 for 30 min, and referred to gyrB as internal control [26]

These elements are crucial as they can transcribe the expression of MTs, particularly by minimizing ROS-mediated cytotoxicity, thus adding another layer of protection against arsenics harmful effects

Cell and region picking and raw image acquisition were performed in a blinded manner

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