2B show that the presence of 2 M PSC-833, a specific inhibitor of P-gp ATPase [33], reverses the collateral sensitivity of CHO R C5 and MDA-Doxo 400 cells to tamoxifen, with shift in IC 50 values from 0.900 0.090 and 3.582 0.048 M to 2.998 0.071 and 4.336 0.895 M with PSC-833 for CHO R C5 and MDA-Doxo 400 , respectively
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Cell membranes were solubilized in solubilization buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 1% sodium cholate, 0.05% DDM, 5 mM MgCl 2 , 2 L CIP, 5 mM -ME, 10 M GDP, 10% glycerol, 0.2 g/ml leupeptin and 150 g/ml benzamidine
Participants on the 5mg dose lost an average of 16.0% of their body weight
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