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In 24 hpi trophozoite-stage parasites, hemoglobin and heme levels were not measurably different, but hemozoin levels were significantly decreased in resistant parasites compared to sensitive parasites (Fig 2B), indicative of decreased turnover of heme into hemozoin in resistant parasites over the preceding 24 h of the parasite lifecycle

RNA was extracted from snap frozen tissue and real-time qRT-PCR performed as described previously 15

CoA deficiency is expected to cause substantial abnormalities in cellular metabolism and can potentially explain the additional 9% weight loss in Cse / mice on the no-Cys diet compared to no-Trp + BSO diet (Fig

10.1038/s41591-024-03453-1 346 NusratS.GulickE.LevinthalD.BielefeldtK

As a result, glutathione remains ineffective as it is not enough to fight off oxidative stress