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The next day, samples were incubated with Alexa Fluor 488-conjugated anti-IgG secondary antibodies and Alexa Fluor 647-conjugated anti-IgG for 1 h and stained with DAPI for 7 min

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Nuclear NAD + is generated by nuclear NMNAT1 and is largely utilized by PARPs for DNA damage response and nuclear sirtuins such as SIRT1, SIRT6 or SIRT7 for epigenetic deacetylation 82,86