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These side effects often follow a pattern, with symptoms frequently occurring after injections and during dose increases

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Although these iPSC-derived systems provide a patient-specific platform for drug discovery and disease investigation, there are multiple limitations in the model that need to be addressed before the iPSC-organoid model can significantly decrease the need for in vivo models

For instance, investigations into defective immunometabolism in CF macrophages have utilized the Seahorse Extracellular Flux analyzer, revealing impaired oxygen consumption, both at baseline and following infection with challenging pathogens like Burkholderia cenocepacia (Hamilton et al., 2021)