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The replacement of the media is essential for removing the CuCl 2 and Cu(OAc) 2 interference before assaying the LDH levels as they are shown to reduce the fluorescence signal read-out within treated wells

Researchers interested in investigating this topic should adhere to the aforementioned recommendations of 2mg/daily, subcutaneously for 15 days

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Although not inherently toxic, extended treatment with ASMswhich is common for patients suffering from a chronic disease like epilepsycan lead to the production and accumulation of toxic secondary metabolites inside the cells, leading to side effects like allergic reactions, cell death and organ damage in severe cases 6

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