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Preparation of plasmids and site-directed mutated proteins KRAS (NM_004985.4) and FBXO28 (NM_015176.4) were cloned into pLV3-FLAG pIRES-FLAG-S and pLV5-HA vectors, respectively

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When virus accumulation was determined in the above mentioned lines we found that TMV titers are the highest in SA-deficient NahG tobacco and the lowest in GSH overproducer plants (CEMK-9 and TRI-2), while F 1 hybrids from CEMK-9 X NahG and TRI-2 X NahG crosses display intermediate virus levels similar to those in wild type cv

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Nov-Dec 2005;12(6):562-572