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Determination of serum biochemical parameters Serum BUN (mg/dl), creatinine(mg/dl), uric acid(mg/dl), Ca (mg/dl), P(mg/dl) and total protein (g/dl) were measured at the end of the study by an enzymatic colorimetric assays with a DIRUI (CS-T240, China) auto-clinical chemistry-analyzer and commercial diagnostic kit Biosystem company, Spain

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Disadvantages: No topical cosmetic applications, not naturally occurring in plasma

After incubation, cells were harvested and washed with PBS in order to remove excess drug

Appetite changes: Because these drugs affect how full you feel, its possible to eat less than usual which is desired for weight loss, but may require monitoring to ensure adequate nutrition