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In addition, to confirm that REW did not induce formation of higher order assemblies followed by complement activation in the absence of cognate antigen, the antibodies were incubated in NHS at 37 C at a concentration of 100 g/mL before formation of IgG complexes and C4d was measured in ELISA (Fig
Among field techniques, bioelectrical impedance analysis (BIA) and non-diagnostic ultrasound evaluation are widely used in clinical practice due to their non-invasive nature, ease of use, and low cost [72, 73]
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