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Relative band pixel intensities were semiquantitatively analyzed using ImageJ software (version 1.8.0, National Institutes of Health, Bethesda, MD, United States), and protein loading was normalized using an antibody against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (sc-32233, Santa Cruz Biotechnology, Santa Cruz, CA)

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4) Blood Disorders Certain conditions affecting blood and bone marrow including some leukaemias and myeloproliferative disorders such as polycythaemia vera can cause elevated B12 due to increased production of B12binding proteins by white blood cells

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Follow-up reports indicate stabilization of cartilage degradation and, in some cases, improvement of joint cartilage