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The effect was attributed to microbial metabolites that promoted a more youthful systemic environment (for example, boosting the production of certain short-chain fatty acids and reducing pro-inflammatory cytokines) [121]

J Immunol 166:33693376

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Then 200 ul aliquot was taken into a small tube, sealed and kept at +4 C for additional time interval of 8 to 15 hours followed by transferring into 20 C freezer