The natural occurrence of anandamide is minimal, and it has a brief duration of activity because of the enzymatic action of fatty acid amide hydrolase (FAAH), which breaks it down into free arachidonic acid and ethanolamine [25,26]
Figure 4 3.6 CB-839 alone has limited effects on primary CLL cells To evaluate the effect of CB-839 monotherapy on primary CLL cells, lymphocytes were isolated from 11 patient samples and incubated with 100 nM, 1 M, 3 M, and 5 M of CB-839 for 24 hours
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Western Blot Cells were lysed using the RIPA lysis buffers system combined with a proteinase inhibitor (Applygen, China)
Public Health Reports, June 26, 1914