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The finding was further supported by c-met-binding experiments, in which HGF was pre-treated with GSSSG and LASSS at the same molar ratio 1:4000 at which the satellite-cell activation index was rescued to a level comparable to that of non-nitrated regular HGF, as shown in Figure 1d, followed by exposure to peroxynitrite (1:2000) and evaluated for c-met-binding by a sandwich ELISA-like assay on recombinant c-met-Fc chimera (Figure 1e, left column
IGF-1 LR3 Protocol Formats IGF-1 LR3 is only used as an injection
Furthermore, it might have diminished the expression of urea cycle enzymes, possibly restored nitrogen balance, and, in theory, altered or improved nitrogen concentrations in different organs
Butylated hydroxytoluene (BHT) (1 mM) was used as a positive control
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