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doi: 10.1016/0024-3205(86)90522-9

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10.1016/j.neulet.2013.11.018 Neurosci

To construct the modified homozygous strain expressing GAP1 as the counter-selectable marker in the BY4743 background, the KanMX fragment was amplified from pFA6A-KanMX6 [32], and HS fragments for pGAP1 were amplified from yeast genomic DNA and these fragments plus pGSH1 (above) assembled into pRS315 to give pRS315- HS-KanMX-pGSH1-HS

Traditional Chinese medicine (TCM) has demonstrated unique advantages in the treatment of cardiovascular diseases, which have been reflected in numerous studies