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Notably, a 2014 randomized double-blind study by Hong et al

Maruyama T, Maruyama N, Higuchi T, Nagura C, Takashima H, Kitai M, et al

The Na + -ion binding site is also present in the ligand-free inward-facing structure, with the same occluded binding cavity described above

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At the end of the experiment, the whole fish in each tank were anthesthetized on ice, and sampled to collect liver, muscle and visceral for molecular and biochemical indexes