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Additionally, trehalose is effective against freezing and dehydration, making it a valuable protective agent during drought conditions
On the next day, targeting plasmids (sgRNA or overexpression) were co-transfected with pMD2.G (#12259, Addgene) and psPAX2 (#12260, Addgene) plasmids at 1:1:1 molar ratio using Lipofectamine 2000 (#11668019, Thermo Fisher) at 1:2.5 ratio
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Duplications in plant genomes could be either as small-scale such as tandem and segmental duplications, or as large-scale such as whole-genome duplications (Ramsey and Schemske, 3)
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