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coli BL21(DE3) using standard IPTG induction and purified by Immobilized metal affinity chromatography (IMAC) followed by Size exclusion chromatography (SEC) using a Superdex75 16/60 column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.4, 150 mM NaCl, 5 % (v/v) glycerol

Storage conditions: The raw materials should be stored away from light in an inert atmosphere at -20C to prevent moisture absorption and chemical degradation

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Researchers should accordingly source their BPC-157 and TB-500 blend from a reputable vendor with transparent sourcing processes, third-party lab testing, and ethical marketing

And here, the BPC-157 comes into play