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A lactam bridge loaded at Glu 22 , either c[Lys 18 Glu 22 ] or c[Glu 22 Lys 26 ], shows lower affinity and activity than a simple substitution of Gly 22 with Glu.28,36 Theoretically, the substitution of Gly 22 with Ala would make the -helix more stable, but increments in the affinity and activity were not found

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Our study showed that CuCl 2 and Cu(OAc) 2 had significantly upregulated HaCaT cell expression of IL-1 and IL-8 at both the gene level and protein level

( B and C ) Plasma DPP-4 activity just before (time 30 minutes), and at 30 (time 0), 60, 120, and 180 minutes after oral gavage or water of sitagliptin, as indicated ( B , n = 68/group)