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Cells were suspended and lysed in NP-40 lysis buffer supplemented with 5% protease and phosphatase inhibitors (Beyotime, P1046)
Replace proper diagnosis
SLC1A5 might not be an appropriate target for suppressing glutamine uptake by cancer cells because it is not the only plasma membrane glutamine transporter, and its function would therefore be compensated by other redundant glutamine transporters such as SLC38A1 and SLC38A2
Thus, the mutation has survived in the genome in areas where malaria is (or was) prevalent (Africa, Middle East, India, and Southeast Asia)
The gla residues are effective calcium ion chelators
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