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Chromatin fractionation Cells were washed with PBS and lysed in CSK buffer (10 mM PIPES pH 7.0, 100 mM NaCl, 1 mM MgCl 2 , 1 mM EDTA, 300 mM sucrose, 10 mM NaF, 20 mM -glycerophosphate, 100 mM Na 3 VO 4 , 0.5% Triton X-100, and 1 protease inhibitor cocktail)