The cutoff value of H -score was determined as the largest Youden index after analyzing the ROC curve (68.5) (Figure 1D)
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Flow cytometry confirmed the expression of mesenchymal stem cell surface markers including CD29, CD44, CD73, CD90, and CD105, as well as pluripotency markers such as Oct3/4, Sox-2, and SSEA-4 [1]
The viral vector was administered at a rate of 125 nl min 1 using an automatic motorized micropump (Ultramicropump3 micro syringe injector with micro4 controller, World Precision Instruments)