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cDNA is produced by reverse transcription, using a reverse transcription kit (G3333-50, Servicebio), and gene expression levels were measured using ABscript Neo RT master Mix (RK20433, Abclonal)

In nearly all samples, the higher the TOCC, the higher the browning intensity, with the only exception being the samples supplemented with MP in the presence or absence of laccase

Participants were assigned to the 9 mg cohort (12-week treatment with escalation from 3 to 6 to 9 mg) or the 10 mg cohort (16-week treatment with escalation from 2.5 to 5 to 7.5 to 10 mg) in a 2:1 randomization with placebo

Participants received weekly injections of retatrutide or placebo for 48 weeks

The progressive form of MASLD, known as nonalcoholic steatohepatitis (NASH), is characterized by liver inflammation, hepatocellular injury, and fibrosis, and may eventually progress to cirrhosis