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Sections were washed three times with TBST and counterstained with DAPI (5 mg/mL, 1:10,000, Invitrogen) for 5 min to label nuclei

This non-targeted comprehensive analysis of human blood disclosed large-scale shifts in blood metabolite profiles during 58 hr of fasting, comprising significant changes in 46 metabolites, including 32 previously unreported fasting markers (Table 1)

Both cyanocobalamin and methylcobalamin are forms of vitamin B12