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When activated, MC1R triggers a signaling cascade: MC1R activation cAMP increase cAMP activates MITF (master melanocyte transcription factor) MITF upregulates tyrosinase and related enzymes Pheomelanin production shifts to eumelanin production This last point is the key insight

3B, C), validating efficient gene ablation in osteocytes

Following resuspension in 500 l binding buffer, the cells were added with 5 l Annexin V-FITC and 5 l Propidium Iodide (PI) (KeyGene, Nanjing, China) at room temperature in the dark for 15 min