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13 Safety considerations and potential risks of probiotic use 13.1 The need for rigorous strain characterization Although probiotics are widely regarded as safe for the general population, their extensive use across diverse demographic groups, including infants, the elderly, and individuals with compromised immune function, necessitates rigorous and comprehensive safety assessments (53)

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Diluted compounds were administered daily within the vascular perfusion in 3D liver models for 3 days to assess acute toxicity or for 7 days to evaluate prolonged hepatotoxicity (Fig