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[15] The redox potential (E GSH ) was calculated using the following equation: Where E (roGFP2) is the standard redox potential of the fluorescent protein (280 mV, at pH 7), [12] R is the gas constant (8.315 J K -1 mol -1 ), T is the temperature (K), and F is the Faraday constant (96485 C mol -1 )

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Thermo Fisher Scientific, Inc.) were used as primary antibodies

In summary, PSC patients demonstrate distinct changes in gut fungal composition, particularly increases in Candida species and Trichocladium griseum , along with a decrease in Saccharomyces , suggesting that fungal dysbiosis may be associated with PSC pathogenesis