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Buijsse B, Lee DH, Steffen L, Erickson RR, Luepker RV, Jacobs DR Jr, Holtzman JL

The desired monocistronic (single copy) of His6-AV-Ubiquitin- semapeptide (nucleotide sequence)-encoding gene PCR product of ~320bp was obtained as evident by 1% (w/v) Agarose gel electrophoresis (Figure 1)

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One mM of phosphorus weighs 31 mg, and the product provides 93 mg (approximately 3 mM) of phosphorus/mL plus 92 mg (4 mEq) of sodium/mL