though some investigators have reported that heme-induced ROS elicited lipid peroxidation, 4-hydroxy-2-nonenal (4-HNE) adducts, and yet it supported epimastigotes' proliferation (Nogueira et al., 2011)
Both green and red fluorescence signals were recorded, and image analysis was performed using NIS-Elements software (Nikon CEE GmbH, C.R.) for evaluation
[DOI] [PMC free article] [PubMed] [Google Scholar] 59.Perrone L., Mothes E., Vignes M., Mockel A., Figueroa C., Miquel M.-C., Maddelein M.-L., Faller P
Polyhistidine-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture
This is why the clinical picture is almost never simple