Lipofectamine 3000 siRNA transfection reagent (Thermo Fisher Scientific, Carlsbad, CA, USA) was then used to transfect the cells with either negative control or Nrf2 siRNA duplexes at a concentration of 50 nM for 36 h
It is not a peptide, and there is no injectable form with established safety data oral is the right route
Hong S, Moreno-Navarrete JM, Wei X, Kikukawa Y, Tzameli I, Prasad D, Lee Y, Asara JM, Fernandez-Real JM, Maratos-Flier E, Pissios P. Nicotinamide N-methyltransferase regulates hepatic nutrient metabolism through Sirt1 protein stabilization. Nat Med. 2015 Aug;21(8):887-94. doi: 10.1038/nm.3882. Epub 2015 Jul 13. PMID: 26168293; PMCID: PMC4529375
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