Using the TLR7/8 agonist 3M-052 alum as an adjuvant, RBD-scNP was designed to be a pan--coronavirus/merbecovirus vaccine
Materials and methods Chemicals and reagents Cytochrome c, reduced glutathione (GSH), oxidized glutathione (GSSG), 5,5-dithio-bis(2-nitrobenzoic acid), cumene hydroperoxide, dimethylnitrosamine (DMNA), erythromycin, dinitrophenylhydrazine (DNPH), lucigenin, glutathione reductase, thiobarbituric acid, NADH, NADPH, coenzyme Q2, antimycin A, sodium succinate, dodecyl maltoside, Hexokinase assay kits, and ATP Bioluminescent cell assay kits were purchased from Sigma-Aldrich Fine Chemicals (St Louis, MO, United States)
Table S5
Table 1 Role of pro-inflammatory and anti-inflammatory cytokines in wound healing
Protocol suggested by Dhindsa et al