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The toluene layer was transferred to a glass GC/MS vial with a volume reducing insert

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Confocal imaging analysis of transgenic Arabidopsis root cells revealed that VSR1, the key retromer component VPS29 and SNX1, partially colocalized with MVBs labeled by RHA1/ARA7 (conventional members of the Arabidopsis RAB5 family) and RABG3f (a member of the RAB7 family) (Supplementary Fig