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Another transporter, CACT (carnitine-acylcarnitine translocase) is located in the inner mitochondrial membrane and contributes to the exchange of acylcarnitine and longer acylcarnitines for carnitine to ensure acetylcarnitine transfer across the inner mitochondrial membrane [90]

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and Tween 20, 0.05%, v/v) for 90 min under continuous shaking