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Previous studies have shown that the ROS content in oocytes subjected to aging in vitro is time-dependent [35]

[4] The amino acid sequence is Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val

Research-only context for DSIP in laboratory workflows DSIP is used in laboratory settings as a peptide reference input in controlled research workflows that may include analytical chemistry characterization, method development, stability assessments, compatibility checks with assay matrices, and in vitro assay development where a consistent and traceable compound identity is required

doi: 10.1186/s13075-017-1229-9 168 JingXLinJDuTJiangZLiTWangGet al

For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35