[DOI] [PMC free article] [PubMed] [Google Scholar] 59.Gabery S, Salinas CG, Paulsen SJ, Ahnfelt-Ronne J, Alanentalo T, Baquero AF, et al
Beyond sourcing, stability is another factor I always consider very carefully
A minimum three-hour fasting window before injection is critical
Supernatants were collected, and protein concentrations were determined using the BCA protein Assay Kit (Thermo Fisher Scientific, CO)
However, the mechanism likely involves the combination of weight loss, changes in nutritional absorption due to slowed gastric emptying, and potentially reduced loading on bones (since bones respond to mechanical load by increasing density)