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We next used a panel of tumor cell lines from different types of cancers with relatively high ERK5 mRNA or protein levels (based on the DepMap resource and protein level verification by us) [45, 46], namely A427 (lung adenocarcinoma), Huh-7 (hepatocellular carcinoma), JHH-7 (hepatocellular carcinoma) and MDA-MB-231 (breast adenocarcinoma) cells