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All bacteria were incubated at 37C unless otherwise requested

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Further research is needed to elucidate the basic mechanism underpinning T and B-cell recruitment into an immune response, to examine antibody affinity maturation and to identify the determinants of the immunodominance hierarchy for T and B-cell epitopes, which ultimately may enable vaccine antigens to be rationally designed to induce broadly targeted immunity

In the presence of either a carryover effect or period effect, a repeat of the analyses focusing on data from the first period (prior to washout) was planned