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The derivatization protocol for the quantification of TMA was an adaptation of the protocol described by Samples for TMAO levels quantification were prepared as follows: an amount of 25 L of each sample was collected, and 3 L of D9-TMAO (5 M concentration) and 122 L of a cold acetonitrile:methanol mixture (50:50, v/v) were added for protein precipitation

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The cells are harvested for a luciferase assay using a luciferase assay [1]

According to the principles of TCM, combining Astragalus membranaceus with S