Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator
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Thiol-disulfide redox equilibria of glutathione metaboloma compounds investigated by tandem mass spectrometry
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[DOI] [PubMed] [Google Scholar] 84.Chanez P., Burge P.S., Dahl R., Creemers J., Chuchalin A., Lamarca R., Garcia Gil E