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GSH Metabolism, Biosynthesis & Health Implications (PubMed, 2004) Cysteine Availability as the Rate-Limiting Factor in GSH Synthesis A widely cited review of glutathione metabolism published on PubMed documented the two-step cytosolic biosynthesis pathway in detail, confirming that GSH synthesis is regulated primarily by gamma-glutamylcysteine synthetase activity, cysteine availability, and GSH feedback inhibition

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These findings align with previous reports that melatonin reduces ILC2-derived IL-5 and IL-13 in oxazolone-induced colitis models (65), suggesting a conserved anti-inflammatory effect of melatonin across tissues

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Denburg, M