Oxygen consumption rates An hour before oxygen consumption measurements, cell media was replaced by assay media (2 mM glucose, 0.8 mM Mg 2+ , 1.8 mM Ca 2+ , 143 mM NaCl, 5.4 mM KCl, 0.91 mM NaH 2 PO 4 , and 15 mg/mL Phenol red) for 60 min at 37 C (no CO 2 ) before loading into the Seahorse Bioscience XF96 extracellular analyzer 102
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Once-weekly cagrilintide, a long-acting amylin analog that is non-selective for the CTRs and amylin receptors, has shown promising weight-loss effects as well as improvement in cardiometabolic factors
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These include the use of Smoothened (Smo) agonists to activate Shh signaling, as well as small-molecule modulators of intraflagellar transport (IFT) proteins to restore ciliary trafficking and structural integrity [263268]