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2.5 Quantification of oxidative phosphorylation and glycolysis The Seahorse XF96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA, United States) was utilized to measure real-time integrated cellular oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) as previously outlined (Lan et al., 2018

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The complex I and complex III of ETC are able to produce the superoxide anion radical (O 2 ) and release it to both the matrix and the intermembrane space

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