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Subcutaneous administration (which bypasses first-pass metabolism and delivers more peptide to target tissues) was not extensively tested in human trials, and this route is what most current research protocols use

Even low levels of endotoxin contamination can trigger unintended inflammatory signalling in cell-based assays, significantly confounding results

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Neither choice implies any human application

Collectively, these findings suggest that thymosin-derived peptides represent promising tools for exploring neuroprotective mechanisms and adaptive repair processes in experimental neurobiology