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Wells were washed with PBST and incubated for 1h with anti-GSTM1 pAb, then aspirated and washed 3 times

In terms of mechanism, Nrf2-mediated pathways play important roles in the therapeutic effects of SFN, such as Nrf2-mediated inhibition of lipogenesis and oxidative stress, anti-inflammation, and suppression of ER stress, Nrf2-mediated lipophagy, and mitochondrial function

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Jaridon 6, a diterpene derived from Rabdosia rubescens, is thought to have the ability to activate autophagy