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To test the effect of Sp in paraquat-induced oxidative stress model, seven days old wild type flies, constitutively expressing mitochondrial (mt)-roGFP, were exposed to 10 mM paraquat for 24 h and then co-treated with 2.5 mM Sp for 24 h, prior to roGFP fluorescence estimation

But if that vial actually contains 7.79mg (the low end of tested 10mg vials), your actual concentration is 3.895mg/mL, not 5mg/mL

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