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DNA methylation Genomic DNA was isolated from the liver tissue or cultured cells using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germantown, MD) following the manufacturers instructions

(1973b)

Caloric restriction and reduced GH/IGF-I signaling improve resistance to cellular stress and inhibit mTOR, thereby increasing longevity and possibly enhancing tumor resistance

GlucosamineEssential for rebuilding cartilage and connective tissue

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